KMID : 0380620130450040526
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Korean Journal of Food Science and Technology 2013 Volume.45 No. 4 p.526 ~ p.529
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Effects of the 461-nm LED Light and Combination with Acid Stress Treatment on Staphylococcus aureus and Escherichia coli
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Kim Se-Hun
Bang Woo-Suk
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Abstract
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This study was conducted to evaluate the disinfection effects of Staphylococcus aureus, and Escherichia coli treated with 461-nm LED and pH 5 at 15oC for 10 h. S. aureus strains were decreased by about 4 log CFU/mL after 461- nm LED irradiation treatment alone for 10 h. E. coli strains were inactivated by irradiation. However, when microorganisms were subjected to a combined treatment of 461-nm LED and pH 5, both strains were inactivated by irradiation for 7 h. The highest D-value was 5.05 h for S. aureus ATCC 27664 and the lowest D-value was 1.39 h for E.coli O157: H7 ATCC 35150 (p<0.05) with 461-nm LED irradiation. For the combined treatment (461-nm LED and pH 5), the highest D-value was 1.58 h for S. aureus ATCC 19095, whereas the lowest D-value was 0.83 h for S. aureus ATCC 27664 (p<0.05). These data showed that bactericidal effects of a combination of pH 5 with 461-nm LED irradiation were enhanced compared to 461-nm LED irradiation alone.
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KEYWORD
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461-nm LED, combined stress, Staphylococcus aureus, Escherichia coli
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